Choosing suitable primers is one of the most important factors affecting 3 basic steps of the polymerase chain reaction process (PCR).

CRISPR/Cas

Owing to CRISPR/Cas9 technology, speedily expanding gene editing field spawned a lot of CRISPR companies.

Recombinant DNA technology - The Chimera

Genetic Engineering - using recombinant DNA technology to modify an organism’s DNA to achieve desirable traits.

DNA sequencing methods

DNA sequencing is a process of determining the order of the four chemical building blocks - called "nitrogenous bases" - that make up the DNA molecule.

Application of PCR in DNA analysis

PCR stands for Polymerase Chain Reaction which is one of the fundamental methods of molecular biology.

There are a lot of thermocycling and isothermal techniques for amplification of nucleic acids, such as Strand Displacement Amplification, Rolling Circle Amplification, Ligase Chain Reaction, Helicase Dependent DNA amplification, etc.

Application of PCR

In order to gain information about the DNA, different types of the PCR is often the starting point in series of experiments.

PCR components

5 standard PCR components play crucial roles in DNA amplification.

The polymerase chain reaction requires a set of appropriate oligonucleotide primers, DNA template and polymerase, a buffer and deoxynucleotides (dNTPs).

DNA extraction

Biotechnology is the use of artificial methods to modify the genetic material of living organisms or cells to produce novel compounds or to perform new functions.